ANTI-HEPATITIS C ACTIVITY OF EXTRACTED FRACTION OF PSEUDOMONAS OLEOVORANS USING HEPATITIS C VIRUS CELL CULTURE SYSTEM

One of the primary goals antiviral research in recent decades has been biosynthesis new agents. The large number bioactive agents extracted from microbial isolates led to building connections between products through screening. Using a genotype 4 plasmid, extract fraction Pseudomonas oleovorans evaluated against HCV Hepatitis C cell culture. cytotoxicity assay using MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] for determination ability mitochondrial enzyme succinate dehydrogenase breakdown salt and reflect viability indicates inhibitory concentration 50% is732.2 µg/mlto be further applied conventionalin assay. screenedthrough inhibition viral entry, interference with replication, blocking assembly release assays. In both pre-treatment replication assays, inhibited infection culture systems reduction RNA 9,837 <12 4,035 < 12 IU/ml respectively. On other hand, there was slight case inhibiting releasemechanism 4950 3710 after detecting Real-time PCR.